Revealing structural views of biology.
نویسندگان
چکیده
The first protein structures were determined in the 1950s. In the decades that followed, development of new methods for sample preparation, crystallization, data collection, and structure analysis yielded tens of thousands of biomolecular structures. This short review highlights some of the major technical advances exemplified with selected structures.
منابع مشابه
Combining single-molecule manipulation and single-molecule detection.
Single molecule force manipulation combined with fluorescence techniques offers much promise in revealing mechanistic details of biomolecular machinery. Here, we review force-fluorescence microscopy, which combines the best features of manipulation and detection techniques. Three of the mainstay manipulation methods (optical traps, magnetic traps and atomic force microscopy) are discussed with ...
متن کاملGlycation of Human IgG Induces Structural Alterations Leading to Changes in its Interaction with Anti-IgG
Background: Glycation of proteins is a non-enzymatic spontaneous process that occurs in diabetes mellitus and aging, altering the structure and function of proteins. IgG undergoes glycation leading to changes in its reactivity to antigen and fixation of complement. Objective: This study aimed at revealing the effect of glycation on the interaction of IgG with anti-IgG using electroimmunoassay...
متن کاملHammerhead ribozyme structure: U-turn for RNA structural biology.
Two crystal structures of the hammerhead ribozyme provide the first atomic-resolution views of an RNA active site, and suggest that the catalytic center may reside in a U-turn motif which was first seen in tRNA(Phe).
متن کاملProtein folding and three-dimensional domain swapping: a strained relationship?
Many proteins function as multimeric assemblies into which the folded individual promoters organize as higher order structures. An oligomerization mechanism that appears to impose the coordination of events during folding and oligomer assembly is three-dimensional domain swapping. Recent studies have focused on revealing the structural basis of domain swapping and a possible role for domain swa...
متن کاملLigands in crystal structures that aid in functional characterization
Liganded structures can be instrumental in assigning function to uncharacterized proteins by revealing active sites, conserved residues, binding motifs, and substrate specificity. This introduction provides an overview and commentary on the value of liganded structures emerging from the JCSG structural genomics initiative.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Biopolymers
دوره 99 11 شماره
صفحات -
تاریخ انتشار 2013